4-AcO-DET (Ethacetin)

4-AcO-DET (Ethacetin) is a synthetic tryptamine prodrug for serotonin research. Purity ≥98% (HPLC).

**Product Name:** 4-AcO-DET (4-Acetoxy-N,N-diethyltryptamine)

**Synonyms:** Ethacetin; Ethylacybin; 4-Acetoxy-DET; 3-[2-(diethylamino)ethyl]-1H-indol-4-yl acetate

**General Description:** 4-AcO-DET is formally described as 3-[2-(diethylamino)ethyl]-1H-indol-4-yl acetate. It is a synthetic, psychoactive molecule belonging to the tryptamine class. Initially synthesized by Albert Hofmann and Franz Troxler at Sandoz Laboratories in 1958, it is the acetylated ester of 4-HO-DET (ethocin) and a homologue of 4-AcO-DMT (O-acetylpsilocin). In analytical, clinical, and forensic toxicology, 4-AcO-DET is utilized as a reference standard for the calibration of analytical instruments (such as LC-MS/MS, GC-MS, and HPLC) and the development of screening methodologies to identify and quantify illicit designer drugs in seized materials or biological matrices.

**Technical Information:** * **CAS Number:** 1135424-15-5 (Freebase) / 2749504-15-0 (Hydrochloride salt) *

**Molecular Formula:** C₁₆H₂₂N₂O₂ *

**Formula Weight:** 274.36 g/mol (Freebase) *

**SMILES:** CCN(CC)CCC1=CNC2=CC=CC(OC(C)=O)=C21 *

**InChI Key:** ODVLAYUXIAMBFN-UHFFFAOYSA-N *

**4-AcO-DET (Ethacetin) Formulation:** Typically supplied as a neat crystalline solid (often formulated as a fumarate or hydrochloride salt to ensure chemical stability, resistance to oxidation, and accurate dosing) or as a certified reference material solution (e.g., 1 mg/mL in methanol).

**Biological Action:** 4-AcO-DET functions largely as a prodrug to the active central nervous system compound 4-HO-DET. Upon ingestion or systemic administration, the ester rapidly undergoes *in vivo* hydrolysis to yield the free phenol (4-HO-DET). Its pharmacological effects are mediated via agonism or partial agonism at central serotonin receptors, predominantly the 5-HT_2A and 5-HT_2C subtypes.
The robust activation of the 5-HT_2A receptor is principally responsible for mediating its classical psychedelic properties, such as the head-twitch response (HTR) observed in murine behavioral models.

**Regulatory and Safety Information:**
* **Intended Use:** This product is intended strictly for use as an analytical reference standard for forensic, toxicological, and academic research purposes.

* **Warning:** **This product is not for human or veterinary use.**

* **Legal Status:** The regulatory status of 4-AcO-DET varies globally. In the United Kingdom, it is controlled as a Class A substance under the Misuse of Drugs Act. In the United States, 4-AcO-DET is not explicitly listed as a scheduled controlled substance at the federal level; however, because it is an ester of 4-HO-DET and structurally homologous to Schedule I substances such as DET and psilocin, its manufacturing, distribution, or possession can be prosecuted under the Federal Analogue Act if intended for human consumption. Procurement may require relevant local or institutional licenses. Handling should only occur within professional laboratory environments using appropriate Personal Protective Equipment (PPE) such as safety glasses, chemical-resistant gloves, and a laboratory coat.